Author: Zech
Plasmid design and constructionA summary of plasmid constructs are in Supplementary Table 1 and plasmid sequences are in Supplementary Data 2. Unless otherwise specified, cloning was performed by Gibson Assembly of PCR-amplified or commercially synthesized gene fragments (from Integrated DNA Technologies or Twist Bioscience) using NEBuilder Hifi Master Mix (NEB, E262), and final plasmids sequence-verified by Sanger sequencing of the open reading frame and/or commercial whole-plasmid sequencing service provided by Primordium.Protein expression constructsTo summarize, denAsCas12a-KRAB, multiAsCas12a-KRAB, multiAsCas12a and enAsCas12a-KRAB open reading frames were embedded in the same fusion protein architecture consisting of an N-terminal 6xMyc-NLS29 and C-terminal XTEN80-KRAB-P2A-BFP103. The denAsCas12a…
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Being an avid gamer, I love checking out the latest gaming consoles that allow me to experience different things and discover new worlds. The best gaming consoles have amazing graphics and great online capabilities that provide a fun and immersive experience. I love the Sony PlayStation 5 because it’s fast, looks great, and has some great exclusive games. My only pet peeve is its bulky design, but that issue has been addressed with its successor, the PS5 Slim model, which is 30% smaller than the original and has more storage space, but otherwise offers the same experience. Photo: Amazon Photo:…
As the games industry undergoes a rapid technological revolution, it is important to understand the business trends underlying this transformation. From breakthrough innovation to strategic investments, the games industry’s business landscape is evolving to meet the demands of a tech-savvy audience. Here we unravel the complex web of factors shaping the business behind games’ technological revolution and explore the implications for players, developers and investors. Investing in Innovation Investment in research and development is at the core of the gaming industry’s technological revolution. From venture capital funding to strategic partnerships to government grants, industry leaders and startups are employing a…
OpenAI has announced significant improvements to data analysis for ChatGPT with the new GPT-4o model. The update includes several features that streamline the data analysis process for users.Firstly, ChatGPT now enables direct file uploads from Google Drive and Microsoft OneDrive, allowing users to access and analyze data stored in cloud services without having to download and upload files, making the process more efficient and convenient.Once files are uploaded, users can interact with tables and charts in the new expandable view, creating a more immersive analytical experience. The expandable view allows users to stay focused on the data and dynamically interact…
While a slew of recent generative AI tools have wowed people with their ability to generate human-like output, many users have found over time that they aren’t actually all that beneficial in terms of covering all aspects of manual tasks. You still need to understand what you’re asking these systems to do to ensure information accuracy, and you also need basic process knowledge to maximize their value. But for many manual tasks, ChatGPT and other tools will be transformative once people fully understand how to incorporate them and integrate them functionally. And in that respect, this could be a big…
The AI boom is taking all the stops, and satellite data analytics is the next step. Downstream space segments are facing an urgent need to adopt AI and drive their offerings to meet the next generation needs of their customers. But will it last or is it just hype? To discuss these points, a session entitled “Convergence of technologies to drive the downstream space segment” was held on the third day of the Geospatial World Forum 2024. Moderated by Novaspace Manager Alexis Conte, the session discussed new-age technology and how it can benefit society and help solve modern-day problems. Data…
On Wednesday, May 15, 2024, a bipartisan US Senate working group led by Majority Leader Sen. Chuck Schumer (D-NY) released a report titled “Driving U.S. Innovation in Artificial Intelligence: A Roadmap for Artificial Intelligence Policy in the United States Senate.” The report follows a series of off-the-record “educational briefings,” a classified briefing, and nine “AI Insight Forums” hosted in the fall of 2023 that drew on the participation of more than 150 experts from industry, academia, and civil society.While some industry voices voiced praise for the report and its recommendations – for instance, IBM Chairman and CEO Arvind Krishna issued…
Luke Conroy and Anne Ferres & AI4Media / AI Better Images / Models Built from Fossils / CC-BY 4.0Generative artificial intelligence (AI) tools like OpenAI’s ChatGPT, Stability AI’s Stable Diffusion, and Midjourney have repeatedly sparked waves of panic and excitement since their release. News reports have understandably focused on how these tools make it easier and cheaper to create high-quality synthetic media, including images, video, and audio, and distribute it at scale in multiple languages with hyper-local targeting.These tools, if misused, have the potential to dramatically destabilize reality. Technologists and scholars are already using the phrase “lying dividend” – the…
In response to U.S. President Joseph Biden’s Executive Order 14081, “Advancing Biotechnology and Biomanufacturing Innovation for a Sustainable, Safe, and Secure U.S. Bioeconomy,” the U.S. Environmental Protection Agency (EPA), U.S. Food and Drug Administration (FDA), and U.S. Department of Agriculture (USDA) have developed plans to update, streamline, and clarify regulatory and oversight mechanisms for biotechnology products.Executive Order 14081, issued on September 12, 2022, aims to accelerate biotechnology innovation and grow America’s bioeconomy across multiple sectors, including health, agriculture, and energy. The Executive Order seeks to support the safe use of biotechnology products by clarifying and streamlining regulations to achieve a…